The transfer curve is a plot of the square of the noise (variance) in an image after correction for flat-field effects versus the mean counts. It was first developed as a tool for investigation of CCD properties by Janesick (1987) and is used to measure the CCD conversion factor in electrons/ADU.
At illumination levels less than saturation, the variance of the
signal in electron units,
is given by:

where
is the noise in the signal and
is
the readout noise in electrons. Assuming Poisson statistics,
is the signal in electrons and is equal to the
illumination level in electrons,
which is the illumination
level in ADU,
, multiplied by the CCD conversion factor (or,
imprecisely, the gain), g, in electrons/ADU. Similarly,
is the standard deviation of the signal in ADU,
, multiplied by g. Thus:

or

Therefore, the slope of the plot of the variance of the signal in ADU,
, versus the mean counts,
, is the inverse of
the CCD conversion factor.
The readout noise is obtained from the square root of the y-intercept, which is simply the variance of the signal in a bias frame, divided by the conversion factor.
Note: at high count levels, the transfer curve levels off and may turn over. This is because the system has become saturated. Saturation may be defined in either of two ways.
Normally, the system gain should be set so that digital saturation is encountered at signal levels less than analogue saturation.